The environmental factors that effect turnip peroxidase Essays

The environmental factors that effect turnip peroxidase Essays The environmental factors that effect turnip peroxidase Paper The environmental factors that effect turnip peroxidase Paper The changing of pH, substrate concentrations, temperature, and an inhibitor (Niacin) and the effects it hade on the enzyme turnip peroxides. Enzymes are biological catalysts which increase reaction rates by lowering the activation energies of substrates. A substrate is a reactant that interacts with the enzyme. The enzyme and substrate can be viewed as the recently discovered induced fit model, which suggests enzymes are flexible and dynamic things that change their shape so all these substrates (reactants) can become catcalled when the activation energy is lowered and the reactions happen a lot faster. Sometimes in cells though it may not need a particular substrate (reactant) so an inhibitor comes into play. Inhibitors are basically regulators that inhibit (disallow) the process of catalysis to take place within a particular substrate. There are two types of inhibitors that occur in such reactions. Competitive inhibitors are remarkably similar to the substrate, so much that it can match interchangeably with the substrate, thus leading to a halt in the production of the intended product. Another variety of inhibitor is the electric inhibitors. Electric inhibitors change the shape of the enzyme by binding to a different site other the active site, which is the usual site for catalysis. Usually the electric inhibitors make contact with the side of the enzyme opposite the active site. In some occasions however an electric activator is introduced and functions by connecting to the enzyme in a way that it allows for easy access for a substrate to the active site. Electric activators are the opposite of electric inhibitors. These regulators (both inhibitive and active in function) help keep the cell in homeostasis by not allowing too much or too little of a added or not needed product to be produced.. Enzyme activity is also regulated by cofactors which are either metal ions (e. G. Zen+, MGM+) or small organic molecules (e. G. Commence A, AND, NADIA, and many vitamins in our diet like iron). Cofactors main is to work together with enzymes to enhance the catalytic activity and by stabilizing the transition state. The transition state is the second of three steps of catalytic action of an enzyme. The transition state and termination state are important in regards to cofactors because the cofactors allow interactions between the enzyme and substrate lowering the activation energy required. The goal of the following paper is to inform you the reader on how environmental conditions such as temperature, pH, salt (an electrolyte), and how substrate concentration itself effects the rate of reaction and properties of turnip peroxides. If optimal rates of reaction can be determined for each parameter, perhaps a farmer can grow his turnips more effective and efficiently rather than if he didnt know that surrounding temperature, pH, cofactors, and inhibitors can effect the outcome of how much crop yielded. Turnip peroxides may be insignificant to us because its just a part of a urn but we must realize that the following experiments can and do affect us just as much if not more than these lifeless turnips. Inside our bodies right now things are being catcalled and pH. Enrapture, the amount of a particular substrate, and the presence of electrolytes (or absence of) can effect how we function from day to day. For instance if you over eat if you over eat, your body can only release so many digestive enzymes to break down this food (which can be seen as the substrate) so rate of reaction doesnt increase because their isnt enough digestive enzymes to digest this ridiculous amount of food. Thus an increase a digestive enzym es can catalyst more food leading to higher rate of reaction and less of a tummy ache. So the wise choice would be to eat small portions of food (substrate) so the digestive enzymes can breakdown evenly the food and produce energy for the body rather than wasting energy to breakdown the large amount of food. Thus the common hypothesis is that an increase in substrate concentration will effect peroxides activity. The null hypothesis is that the concentration of substrate will no effect on activity. PH is derived from the French puissant d hydrogen, or power of hydrogen . (biological science). The pH scale is a scale that indicates whether a substance is acidic or basic. The scale ranges from pH zero (acidic) to pH 14(basic). An example of an acidic product is lemon juice and milk of magnesia would be a base. Products are considered acidic because they give up protons during chemical reactions while basic receive protons. (Biological science). Neutral 7 is the point of reference. An example of a neutral solution would be rain water. Knowing the natural pH of turnip peroxides would be interesting to see whether its acidic or basic and to see what affect the two actors (acidic or basic) have on rate of reaction. The following information led the group to believe that the effect of pH activity will increase or decrease the natural pH of turnip peroxides and effect activity. The null hypothesis is that pH will have no effect on peroxides activity. The effect of temperature on an enzyme depends on the plant. Some plants can exist and grow hot climate like the desert and others can grow in cold climates respectively. Plants are said to be incapable of adjusting to internal temperature so it must be flexible to allow growth to occur when the Enrapture outside is optimal. To figure out optimal temperature for turnip peroxides could lead to better methods of choosing climates for the harvest of these turnips. An important term to know when it comes to temperature and the protein property of the peroxides is the term denatured. Denomination OCCUrs when an enzyme (with protein properties) is misfiled and rendered inactive. High temperatures usually lead to denominations because the increase in temperature essentially cooks the protein in the peroxides. Thus the increase in temperature will either increase peroxides activity of hinder it. The null hypothesis would that temperature will not effect activity. Salt is an electrolyte. Meaning the salt molecules (Niacin) will dissolve completely in water and individual and opposite charges will be a byproduct. Thus the amino acid property of peroxides can be affected by the An+ and CLC- ions. Denomination can occur is exposed to a high concentration of salt. Knowing the natural salt content of the turnip peroxides and adding more or less quantities can tell us when the peroxides becomes highly active and eventually denatured. The concentration of salt effects peroxides activity. The null hypothesis is that it doesnt. The equipment needed to test the parameters of the enzyme activity include a spectrophotometer set at Mann, civets, pipettes with pipettes , pipette tips, paraffin squares, blender, Kim wipes. To get the spectrophotometer ready to read our reaction, we need to first set the wavelength to Mann. Then set front left knob to 0%(no light). Insert the reference blank (with water) then set to 100%(no light block). Hen finally you can insert the sample tube into the chamber and red absorbent from the lower scale and the front of the spectrophotometer. Note: be sure to use Kim wipes on civets to prevent fingerprints that could throw off data). Regents or chemicals needed include hydrogen peroxide (1 percent H2O from 3% stock solution), turnip peroxides, glacial, pH buffers, and NCAA. To obtain turnip peroxides the lab instructor blended g of turnip into mm of water then filtered through a pa filter. T o obtain the correct amount of substrate (h2o) and NCAA, the CIVIC ;c.v. equation was necessary. C equals concentration and v equals volume in the equation. To test the effect the effect of pH on reaction rate we prepared 4 sets of action mixtures that contained glacial, h2o, peroxides. And 4 civets containing P HAS 2, 5, 7, and 10. (Note: mix peroxides last because the reaction happens immediately). The cavetti with h2o and glacial are mixed and added to a blank. The pH 2 is then poured in the blank as well. Then finally the peroxides is added. With paraffin covered over the cavetti the mix is inverted a couple of time to mix. The solution will turn brown due to the loss of hydrogen of glacial. Immediately after mixture insert the civets into already set spectrophotometer. Once set begin recording absences (start at zero) every 15 seconds until three minutes have elapsed-after reading values for three minutes discard the mixture appropriately in the waste beaker and clean civets. Now you can test PHS 5,7, 10. To test the effect of substrate concentration on peroxides activity different concentrations of substrate, peroxides glacial and h2o (the substrate) are needed. The procedures of adding chemicals (different amounts for h2o) and peroxides apply. Begin recording absorbency (start at zero) every 15 seconds until three minutes have elapsed. After reading values for three minutes discard the mixture appropriately in the waste beaker and clean civets. Unknown can test PHS 5,7,10. To test the effect of temperature the same amounts of peroxides, glacial, h2o, are used instead of using different pHs we used just pH 7. To regulate temperatures we used water/ice baths a 71 degrees Celsius. All solutions but the indicator (glacial) need to be at the temperatures. When ready the tubes sitting in the baths can be mixed (1 cavetti with h2o and pH 7 buffer, glacial, and finally peroxides) and set in the spectrophotometer. Begin recording absorbency (start at zero) every 1 5 seconds until three minutes have elapsed. After reading values for three minutes discard the mixture appropriately in the waste beaker and clean civets To test the effects of NCAA activity simply get 1 ml solutions of and 2 percent concentrations of 5% stock solution using call=c.v.. Follow same instructions as done for the temperature experiment and remember to mix the enzyme last so you get accurate results. Begin recording absorbency (start at zero) every 15 seconds until three minutes have elapsed. After eating values for three minutes discard the mixture appropriately in the waste beaker and clean civets. Figure 1 . Graph of absorbency of tubes in which salt was added to the assays that already contained a pH buffer (2. 1 ml), h2o (. 2 ml), turnip peroxides(l ml) and ecological(. Ml). Discussion PH had the lowest absorbent and had pH 10 had the highest. Thus peroxides is more prone to being a base. In the varying concentration levels of hydrogen peroxide on peroxides activity showed 2% to be the least active while 1% had the highest rate of reaction. This shows that an increase of the OIC Hahn results in a decrease of inactivity due to denomination. In the affect of temperature on enzyme activity as temperature went up so did the reaction but at the expense of denaturing of the peroxides. For the NCAA effect on peroxides activity showed increase for absorbent for all percentages and at 3% of . 1 ml of salt had the greatest absorbent. This shows that an increase or decrease of 3 hundredths of a ml of NCAA solution is going to denature the turnip peroxides. The hypothesis of each parameter was approved and the null was mulled. NCAA concentration indeed have an effect on the peroxides activity based on the graphs. The temperature did effect whether protein became denatured or not. The higher the pH the more activity occurred in peroxides and showed pH to be optimal for turnip peroxides. The increase of substrate effected the peroxides activity by denaturing the toxic h2o. Some problems were encountered in all the parameters of experimentation. The time between the the transfer of the assays to the spectrophotometeraffects the absorbencys. This could be easily solved by having the assay right by the spectrophotometer to make sure the sample gets in before the enzyme reacts.